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agena massarray methylation analysis  (agena bioscience)


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    agena bioscience agena massarray methylation analysis
    Expression of CTHRC1 correlates with DNA <t>methylation</t> levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena <t>MassARRAY®</t> Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
    Agena Massarray Methylation Analysis, supplied by agena bioscience, used in various techniques. Bioz Stars score: 96/100, based on 2219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/massarray+analysis/Methylation/pmc11319694-62-0-0
    Average 96 stars, based on 2219 article reviews
    agena massarray methylation analysis - by Bioz Stars, 2026-10
    96/100 stars

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    1) Product Images from "CTHRC1 modulates cell proliferation and invasion in hepatocellular carcinoma by DNA methylation"

    Article Title: CTHRC1 modulates cell proliferation and invasion in hepatocellular carcinoma by DNA methylation

    Journal: Discover Oncology

    doi: 10.1007/s12672-024-01194-8

    Expression of CTHRC1 correlates with DNA methylation levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena MassARRAY® Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
    Figure Legend Snippet: Expression of CTHRC1 correlates with DNA methylation levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena MassARRAY® Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Techniques Used: Expressing, DNA Methylation Assay, Methylation, Quantitative RT-PCR, Western Blot

    Related Articles

    Mutagenesis:

    Article Title: Loci Associated With Susceptibility to Biliary Atresia-A Genome-Wide Association Study in Taiwan.
    Article Snippet: es and 104 females) and 558 nonBA controls (275 males and 283 females) for this GWAS analysis using the Axiom GenomeWide TWB 2.0 Array with 686,463 singlenucleotide polymorphisms (SNPs). The MassARRAY Sequenom analysis was then applied to validate the identified top SNPs. Results: We identified rs139120621 (odds ratio [OR] = 17.31, p = 2.78 × 10−9) at 8q22.1, which is mapped to the FLJ46284 gene, s

    Battery:

    Article Title: Loci Associated With Susceptibility to Biliary Atresia-A Genome-Wide Association Study in Taiwan.
    Article Snippet: es and 104 females) and 558 nonBA controls (275 males and 283 females) for this GWAS analysis using the Axiom GenomeWide TWB 2.0 Array with 686,463 singlenucleotide polymorphisms (SNPs). The MassARRAY Sequenom analysis was then applied to validate the identified top SNPs. Results: We identified rs139120621 (odds ratio [OR] = 17.31, p = 2.78 × 10−9) at 8q22.1, which is mapped to the FLJ46284 gene, s

    Real-time Polymerase Chain Reaction:

    Article Title: Loci Associated With Susceptibility to Biliary Atresia-A Genome-Wide Association Study in Taiwan.
    Article Snippet: es and 104 females) and 558 nonBA controls (275 males and 283 females) for this GWAS analysis using the Axiom GenomeWide TWB 2.0 Array with 686,463 singlenucleotide polymorphisms (SNPs). The MassARRAY Sequenom analysis was then applied to validate the identified top SNPs. Results: We identified rs139120621 (odds ratio [OR] = 17.31, p = 2.78 × 10−9) at 8q22.1, which is mapped to the FLJ46284 gene, s

    Extraction:

    Article Title: Loci Associated With Susceptibility to Biliary Atresia-A Genome-Wide Association Study in Taiwan.
    Article Snippet: es and 104 females) and 558 nonBA controls (275 males and 283 females) for this GWAS analysis using the Axiom GenomeWide TWB 2.0 Array with 686,463 singlenucleotide polymorphisms (SNPs). The MassARRAY Sequenom analysis was then applied to validate the identified top SNPs. Results: We identified rs139120621 (odds ratio [OR] = 17.31, p = 2.78 × 10−9) at 8q22.1, which is mapped to the FLJ46284 gene, s

    DNA Extraction:

    Article Title: Loci Associated With Susceptibility to Biliary Atresia-A Genome-Wide Association Study in Taiwan.
    Article Snippet: es and 104 females) and 558 nonBA controls (275 males and 283 females) for this GWAS analysis using the Axiom GenomeWide TWB 2.0 Array with 686,463 singlenucleotide polymorphisms (SNPs). The MassARRAY Sequenom analysis was then applied to validate the identified top SNPs. Results: We identified rs139120621 (odds ratio [OR] = 17.31, p = 2.78 × 10−9) at 8q22.1, which is mapped to the FLJ46284 gene, s

    Polymerase Chain Reaction:

    Article Title: Loci Associated With Susceptibility to Biliary Atresia-A Genome-Wide Association Study in Taiwan.
    Article Snippet: es and 104 females) and 558 nonBA controls (275 males and 283 females) for this GWAS analysis using the Axiom GenomeWide TWB 2.0 Array with 686,463 singlenucleotide polymorphisms (SNPs). The MassARRAY Sequenom analysis was then applied to validate the identified top SNPs. Results: We identified rs139120621 (odds ratio [OR] = 17.31, p = 2.78 × 10−9) at 8q22.1, which is mapped to the FLJ46284 gene, s

    Mass Spectrometry:

    Article Title: Loci Associated With Susceptibility to Biliary Atresia-A Genome-Wide Association Study in Taiwan.
    Article Snippet: es and 104 females) and 558 nonBA controls (275 males and 283 females) for this GWAS analysis using the Axiom GenomeWide TWB 2.0 Array with 686,463 singlenucleotide polymorphisms (SNPs). The MassARRAY Sequenom analysis was then applied to validate the identified top SNPs. Results: We identified rs139120621 (odds ratio [OR] = 17.31, p = 2.78 × 10−9) at 8q22.1, which is mapped to the FLJ46284 gene, s

    Clinical Proteomics:

    Article Title: Loci Associated With Susceptibility to Biliary Atresia-A Genome-Wide Association Study in Taiwan.
    Article Snippet: es and 104 females) and 558 nonBA controls (275 males and 283 females) for this GWAS analysis using the Axiom GenomeWide TWB 2.0 Array with 686,463 singlenucleotide polymorphisms (SNPs). The MassARRAY Sequenom analysis was then applied to validate the identified top SNPs. Results: We identified rs139120621 (odds ratio [OR] = 17.31, p = 2.78 × 10−9) at 8q22.1, which is mapped to the FLJ46284 gene, s

    Variant Assay:

    Article Title: Loci Associated With Susceptibility to Biliary Atresia-A Genome-Wide Association Study in Taiwan.
    Article Snippet: es and 104 females) and 558 nonBA controls (275 males and 283 females) for this GWAS analysis using the Axiom GenomeWide TWB 2.0 Array with 686,463 singlenucleotide polymorphisms (SNPs). The MassARRAY Sequenom analysis was then applied to validate the identified top SNPs. Results: We identified rs139120621 (odds ratio [OR] = 17.31, p = 2.78 × 10−9) at 8q22.1, which is mapped to the FLJ46284 gene, s

    Control:

    Article Title: Loci Associated With Susceptibility to Biliary Atresia-A Genome-Wide Association Study in Taiwan.
    Article Snippet: es and 104 females) and 558 nonBA controls (275 males and 283 females) for this GWAS analysis using the Axiom GenomeWide TWB 2.0 Array with 686,463 singlenucleotide polymorphisms (SNPs). The MassARRAY Sequenom analysis was then applied to validate the identified top SNPs. Results: We identified rs139120621 (odds ratio [OR] = 17.31, p = 2.78 × 10−9) at 8q22.1, which is mapped to the FLJ46284 gene, s



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    Expression of CTHRC1 correlates with DNA <t>methylation</t> levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena <t>MassARRAY®</t> Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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    Image Search Results


    Expression of CTHRC1 correlates with DNA methylation levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena MassARRAY® Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Journal: Discover Oncology

    Article Title: CTHRC1 modulates cell proliferation and invasion in hepatocellular carcinoma by DNA methylation

    doi: 10.1007/s12672-024-01194-8

    Figure Lengend Snippet: Expression of CTHRC1 correlates with DNA methylation levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena MassARRAY® Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Article Snippet: Agena MassARRAY® Methylation Analysis assessed the methylation level of CTHRC1 in the promoter region.

    Techniques: Expressing, DNA Methylation Assay, Methylation, Quantitative RT-PCR, Western Blot